CIL: 51837, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...

• Main Author: Buckles, Thomas
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: Cell polarity establishment and maintenance; RAW 264.7 TIB-71; Mus musculus; Whole cell; Orca
• Online Access: https://dx.doi.org/10.6075/j0sx6cpp; https://library.ucsd.edu/dc/object/bb5982366n

Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : A novel cell population analysis to quantify both initial and long-term drug effects, namely pseudopod adaptation and recovery following an initial drug-induced perturbation, adding drug slowly in 1/4 increments over 90 minutes. ...