CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...

populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at...

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Bibliographic Details
Main Author: Buckles, Thomas
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2022
Subjects:
Online Access:https://dx.doi.org/10.6075/j0st7p8c
https://library.ucsd.edu/dc/object/bb2432819g
id ftdatacite:10.6075/j0st7p8c
record_format openpolar
spelling ftdatacite:10.6075/j0st7p8c 2024-02-04T10:03:42+01:00 CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... Buckles, Thomas 2022 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0st7p8c https://library.ucsd.edu/dc/object/bb2432819g unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil50994 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 RAW 264.7 TIB-71 Whole cell Cell polarity establishment and maintenance Mus musculus Dataset dataset 2022 ftdatacite https://doi.org/10.6075/j0st7p8c10.7295/w9cil5099410.6075/j0r20zxn 2024-01-05T01:30:46Z populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology)
institution Open Polar
collection DataCite Metadata Store (German National Library of Science and Technology)
op_collection_id ftdatacite
language unknown
topic RAW 264.7 TIB-71
Whole cell
Cell polarity establishment and maintenance
Mus musculus
spellingShingle RAW 264.7 TIB-71
Whole cell
Cell polarity establishment and maintenance
Mus musculus
Buckles, Thomas
CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
topic_facet RAW 264.7 TIB-71
Whole cell
Cell polarity establishment and maintenance
Mus musculus
description populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ...
format Dataset
author Buckles, Thomas
author_facet Buckles, Thomas
author_sort Buckles, Thomas
title CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
title_short CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
title_full CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
title_fullStr CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
title_full_unstemmed CIL: 50994, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
title_sort cil: 50994, mus musculus, raw 264.7 tib-71. in cell image library ...
publisher UC San Diego Library Digital Collections
publishDate 2022
url https://dx.doi.org/10.6075/j0st7p8c
https://library.ucsd.edu/dc/object/bb2432819g
genre Orca
genre_facet Orca
op_relation https://dx.doi.org/10.7295/w9cil50994
https://dx.doi.org/10.6075/j0r20zxn
op_rights Creative Commons Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/legalcode
cc-by-4.0
op_doi https://doi.org/10.6075/j0st7p8c10.7295/w9cil5099410.6075/j0r20zxn
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