CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at...
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2022
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Online Access: | https://dx.doi.org/10.6075/j0rx9bgd https://library.ucsd.edu/dc/object/bb29106385 |
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ftdatacite:10.6075/j0rx9bgd 2024-02-04T10:03:42+01:00 CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... Buckles, Thomas 2022 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0rx9bgd https://library.ucsd.edu/dc/object/bb29106385 unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil50970 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Cell polarity establishment and maintenance Mus musculus RAW 264.7 TIB-71 Whole cell Dataset dataset 2022 ftdatacite https://doi.org/10.6075/j0rx9bgd10.7295/w9cil5097010.6075/j0r20zxn 2024-01-05T01:15:57Z populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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unknown |
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Cell polarity establishment and maintenance Mus musculus RAW 264.7 TIB-71 Whole cell |
spellingShingle |
Cell polarity establishment and maintenance Mus musculus RAW 264.7 TIB-71 Whole cell Buckles, Thomas CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
topic_facet |
Cell polarity establishment and maintenance Mus musculus RAW 264.7 TIB-71 Whole cell |
description |
populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... |
format |
Dataset |
author |
Buckles, Thomas |
author_facet |
Buckles, Thomas |
author_sort |
Buckles, Thomas |
title |
CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_short |
CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full |
CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_fullStr |
CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full_unstemmed |
CIL: 50970, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_sort |
cil: 50970, mus musculus, raw 264.7 tib-71. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2022 |
url |
https://dx.doi.org/10.6075/j0rx9bgd https://library.ucsd.edu/dc/object/bb29106385 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil50970 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6075/j0rx9bgd10.7295/w9cil5097010.6075/j0r20zxn |
_version_ |
1789971310154088448 |