CIL:31926, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: phase contrast microscopy Parameter imaged: elastic scattering of photons Source of contrast: differences in amount of elastic light scattering Visualization methods: visuali...

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Bibliographic Details
Main Authors: Hatano, Toshiyuki, Sluder, Greenfield
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
Online Access:https://dx.doi.org/10.6075/j0rj4hcf
https://library.ucsd.edu/dc/object/bb74134437
Description
Summary:Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: phase contrast microscopy Parameter imaged: elastic scattering of photons Source of contrast: differences in amount of elastic light scattering Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software. ...