CIL:40364, Sus scrofa domestica, kidney epithelial cell. In Cell Image Library ...

• Main Authors: Ferenz, Nick, Wadsworth, Patricia
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: microtubule cytoskeleton organization; mitotic metaphase; kidney epithelial cell; LLC-PK1; microtubule cytoskeleton; Sus scrofa domestica; mitosis; Orca
• Online Access: https://dx.doi.org/10.6075/j0r210r6; https://library.ucsd.edu/dc/object/bb6287304p

Time series images of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at metaphase in a vertical strip between the metaphase plate and spindle pole. The activated strip moves poleward, demonstrating a marked microtubule flux during mitotic metaphase. A movie prepared from this time series is included in this group of images. ... : Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp. See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002 ...