CIL:41313, Canis lupus familiaris, epithelial cell. In Cell Image Library ...

• Main Authors: Greb, Christoph, Jacob, Ralf, Schu, Anja
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: P-75; primary cilium; Canis lupus familiaris; epithelial cell; MDCK-II; acetylated tubulin; Canis lupus
• Online Access: https://dx.doi.org/10.6075/j0qn66wx; https://library.ucsd.edu/dc/object/bb16802485

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: total internal reflection microscopy (TIRF) Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; distribution of a specific protein Visualization methods: EGFP; primary antibody plus labeled secondary antibody ... : Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details. ...