CIL:41313, Canis lupus familiaris, epithelial cell. In Cell Image Library ...

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: total internal reflection microscopy (TIRF) Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; distribution of a specific protein Visualization metho...

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Bibliographic Details
Main Authors: Greb, Christoph, Jacob, Ralf, Schu, Anja
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
P-75
primary cilium
Canis lupus familiaris
epithelial cell
MDCK-II
acetylated tubulin
Canis lupus
Online Access:https://dx.doi.org/10.6075/j0qn66wx
https://library.ucsd.edu/dc/object/bb16802485
Description
Summary:Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: total internal reflection microscopy (TIRF) Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; distribution of a specific protein Visualization methods: EGFP; primary antibody plus labeled secondary antibody ... : Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details. ...

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