CIL:25303, Drosophila melanogaster. In Cell Image Library ...

Movie of colchicine-induced depolymerization of the metaphase spindle in a Drosophila S2 cell expressing GFP-tubulin. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamat...

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Bibliographic Details
Main Authors: Maresca, Thomas, Salmon, Edward
Format: Dataset
Language:unknown
Published: UC San Diego Library Digital Collections 2021
Subjects:
S2
Online Access:https://dx.doi.org/10.6075/j0qj7g2t
https://library.ucsd.edu/dc/object/bb6525491v
Description
Summary:Movie of colchicine-induced depolymerization of the metaphase spindle in a Drosophila S2 cell expressing GFP-tubulin. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 3 in Maresca and Salmon, 2009 J Cell Biol 184:373-281. ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: differences in adsorption or binding of stain Visualization methods: Green fluorescent proteins from Aequorea Processing history: unprocessed raw data ...