CIL:30568, Drosophila melanogaster, early embryonic cell. In Cell Image Library

• Main Authors: Civelekoglu-Scholey, Gul, Tao, Li, Brust-Mascher, Ingrid, Wollman, Roy, Scholey, Jonathan M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: regulation of mitotic prometaphase; regulation of microtubule-based process; Drosophila melanogaster; Olympus; Orca
• Online Access: https://dx.doi.org/10.6075/j0pg1qk5; https://library.ucsd.edu/dc/object/bb5910965b

Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009). Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68. : A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) during surrounding the prometaphase (phase I and II) spindle. Images show a time series of 45 frames from a live embryo, each with six planes through the z axis. Note the indentation of the lamin-B envelope surrounding the spindle by the centrosomes at the opposite ends of the spindle during prometaphase phase I. This image is original data contributing to Fig. 5 "A lamin-B envelope confers robustness to the steady-state prometaphase spindle by stabilizing it to phenotypic variations in the KLP61F to Ncd ratio" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.