CIL:11840, Potorous tridactylus, epithelial cell. In Cell Image Library ...

• Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: epithelial cell; PAK inhibition; Potorous tridactylus; Pt K1 NBL-3; microtubule polymerization; microtubule; Orca
• Online Access: https://dx.doi.org/10.6075/j0n878hs; https://library.ucsd.edu/dc/object/bb6218229j

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubules in a PtK1 cell injected with the Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 4, A and C of associated paper (J Cell Biol, 161:845-851, 2003). ...