CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...
Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: e...
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2021
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Online Access: | https://dx.doi.org/10.6075/j0n58khv https://library.ucsd.edu/dc/object/bb4921904d |
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ftdatacite:10.6075/j0n58khv 2024-02-04T10:04:09+01:00 CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... Ladinsky, Mark Howell, Kathryn E. 2021 application/zip image/jpeg text/plain https://dx.doi.org/10.6075/j0n58khv https://library.ucsd.edu/dc/object/bb4921904d unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil12951 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Rattus rattus lipid storage secretion lactation smooth endoplasmic reticulum glandular epithelial cell milk secreting cell lipid particle protein binding Mitochondrion trans-Golgi network mammary alveolar cell Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0n58khv10.7295/w9cil1295110.6075/j0r20zxn 2024-01-05T01:18:04Z Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements;intensitiesquantitation ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to GOLPH3 (rabbit polyclonal GMx33) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (10 nm anti-rabbit, 15 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Rattus rattus lipid storage secretion lactation smooth endoplasmic reticulum glandular epithelial cell milk secreting cell lipid particle protein binding Mitochondrion trans-Golgi network mammary alveolar cell |
spellingShingle |
Rattus rattus lipid storage secretion lactation smooth endoplasmic reticulum glandular epithelial cell milk secreting cell lipid particle protein binding Mitochondrion trans-Golgi network mammary alveolar cell Ladinsky, Mark Howell, Kathryn E. CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
topic_facet |
Rattus rattus lipid storage secretion lactation smooth endoplasmic reticulum glandular epithelial cell milk secreting cell lipid particle protein binding Mitochondrion trans-Golgi network mammary alveolar cell |
description |
Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements;intensitiesquantitation ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to GOLPH3 (rabbit polyclonal GMx33) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (10 nm anti-rabbit, 15 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained ... |
format |
Dataset |
author |
Ladinsky, Mark Howell, Kathryn E. |
author_facet |
Ladinsky, Mark Howell, Kathryn E. |
author_sort |
Ladinsky, Mark |
title |
CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_short |
CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full |
CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_fullStr |
CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:12951, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_sort |
cil:12951, rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0n58khv https://library.ucsd.edu/dc/object/bb4921904d |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.7295/w9cil12951 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j0n58khv10.7295/w9cil1295110.6075/j0r20zxn |
_version_ |
1789972187067711488 |