CIL:7623, Rattus rattus, epithelial cell, rat kidney proximal tubule epithelial cell. In Cell Image Library ...

• Main Authors: Farquhar, Marilyn G., Palade, George E.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: lysosome; epithelial cell; rat kidney proximal tubule epithelial cell; clathrin-coated endocytic vesicle; zonula adherens; microvillus; endosome; brush border membrane; occluding junction; Rattus rattus
• Online Access: https://dx.doi.org/10.6075/j0mk6bnt; https://library.ucsd.edu/dc/object/bb4853023f

Preparation: osmium tetroxide fixed tissue Relation to intact cell: microtome-sectioned tissue Item type: film Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: lead salt; phosphotungstic acid Processing history: digitized lantern slide Data qualification: Processed;spatialmeasurements;intensitiesquantitation ... : In this experiment, hemoglobin was used as a mass tracer. The rat received two injections, each of 1 gm of 2 X crystallized bovine hemoglobin in ~5 ml saline, administered intraperitoneally, the first at 24 and the last at 16 hours before collecting kidney specimens. Fixative was perfused into the aorta of the rat (2% osmium tetroxide buffered at pH 7.6 with acetate-veronal). Tissue blocks were stained by placing them for 30 minutes in 1 per cent phosphotungstic acid in absolute ethanol prior to infiltration and were embedded in Epon. Thin sections were stained with lead hydroxide. Micrograph was taken at original magnification of 50,000 X with a Siemens Elmiskop I, operating at 60 or 80 kv, with a double condenser, and a 50 µ objective aperture. Digitization process: conversion from a 1200 dpi tiff, 3832 x 3260 pixels. Original image created on June 11, 1962. Original resource provided as a 3.25 x 4 inch lantern slide. Research conducted at The Rockefeller Institute of Medical Research (New York, NY). ...