CIL:35167, Rattus rattus, podocyte (sensu Diptera), epithelial cell. In Cell Image Library ...

• Main Authors: Cammer, Michael, Novikoff, Phyllis
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: renal system process; Cortex of kidney; actin cytoskeleton; podocyte sensu Diptera; epithelial cell; Rattus rattus
• Online Access: https://dx.doi.org/10.6075/j0k936gq; https://library.ucsd.edu/dc/object/bb3249593s

Formaldehyde fixed rat kidney was cut by Vibratome and incubated with fluorescent labeled phalloidin, a toxin which binds to f-actin filaments. Imaging was performed using a BioRad MRC 600 laser scanning confocal microscope with a Kr/Ar laser. The microscope was a Nikon Diaphot with a 60X or 40X fixed tube length objective. Images of adjacent fields were manually stitched using Adobe Photoshop 2.5 on a Quadra Macintosh computer and may have manual contrast curves applied. Imaging performed at the Image Analysis Facility at the Albert Einstein College of Medicine. ... : Preparation: chemically fixed tissue; vibratome-sectioned tissue Relation to intact cell: chemically fixed tissue Item type: recorded image Imaging mode: single-spot confocal microscopy; fluorescence microscopy Parameter imaged: distribution of a specific protein Source of contrast: fluorescence emission Visualization methods: visualization of contiguous regions; fluorescent labels Processing history: montage Data qualification: Processed;spatialmeasurements ...