CIL: 50773, Drosophila melanogaster. In Cell Image Library ...

• Main Authors: Endow, Sharyn A., Miller, Sara E., Thao Ly, Phuong
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: L1 larval brain; Drosophila melanogaster; Orca
• Online Access: https://dx.doi.org/10.6075/j0k64hgs; https://library.ucsd.edu/dc/object/bb0555658q

We show in this study that brain and intestinal stem cells of Drosophila contain a new type of cytoplasmic protrusion which is enriched in mitochondria and that the mitochondria are probably dependent on microtubules for their distribution. The protrusions form mitochondria-enriched cytoplasmic bridges from the stem cells to their niche that we speculate are important in regulating stem cell quiescence and activation. ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ...