CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...
Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and cation-dependent mannose-6...
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2021
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Online Access: | https://dx.doi.org/10.6075/j0jd4w4m https://library.ucsd.edu/dc/object/bb2055144t |
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ftdatacite:10.6075/j0jd4w4m 2024-02-04T10:04:09+01:00 CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... Ladinsky, Mark Howell, Kathryn E. 2021 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0jd4w4m https://library.ucsd.edu/dc/object/bb2055144t unknown UC San Diego Library Digital Collections https://dx.doi.org/10.1016/s0091-679x(06)79021-5 https://dx.doi.org/10.7295/w9cil38664 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Rattus rattus secretion lipid particle mammary alveolar cell trans-Golgi network Mitochondrion protein binding glandular epithelial cell lactation smooth endoplasmic reticulum milk secreting cell lipid storage Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0jd4w4m10.1016/s0091-679x(06)79021-510.7295/w9cil3866410.6075/j0r20zxn 2024-01-05T01:25:55Z Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and cation-dependent mannose-6-phosphate receptor (an integral membrane protein that localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane; smaller gold particles) staining. Note that trans‚ÄêGolgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid. ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to cation-dependent mannose-6-phosphate receptor (rabbit) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (10 nm anti-rabbit, 15 nm anti-mouse; Ted Pella Inc., Redding, CA) and ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Rattus rattus secretion lipid particle mammary alveolar cell trans-Golgi network Mitochondrion protein binding glandular epithelial cell lactation smooth endoplasmic reticulum milk secreting cell lipid storage |
spellingShingle |
Rattus rattus secretion lipid particle mammary alveolar cell trans-Golgi network Mitochondrion protein binding glandular epithelial cell lactation smooth endoplasmic reticulum milk secreting cell lipid storage Ladinsky, Mark Howell, Kathryn E. CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
topic_facet |
Rattus rattus secretion lipid particle mammary alveolar cell trans-Golgi network Mitochondrion protein binding glandular epithelial cell lactation smooth endoplasmic reticulum milk secreting cell lipid storage |
description |
Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and cation-dependent mannose-6-phosphate receptor (an integral membrane protein that localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane; smaller gold particles) staining. Note that trans‚ÄêGolgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid. ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to cation-dependent mannose-6-phosphate receptor (rabbit) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (10 nm anti-rabbit, 15 nm anti-mouse; Ted Pella Inc., Redding, CA) and ... |
format |
Dataset |
author |
Ladinsky, Mark Howell, Kathryn E. |
author_facet |
Ladinsky, Mark Howell, Kathryn E. |
author_sort |
Ladinsky, Mark |
title |
CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_short |
CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full |
CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_fullStr |
CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:38664, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_sort |
cil:38664, rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0jd4w4m https://library.ucsd.edu/dc/object/bb2055144t |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.1016/s0091-679x(06)79021-5 https://dx.doi.org/10.7295/w9cil38664 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j0jd4w4m10.1016/s0091-679x(06)79021-510.7295/w9cil3866410.6075/j0r20zxn |
_version_ |
1789972193089683456 |