CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ...
Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment Relation to intact cell: microtome-sectioned tissue Imaging mode: illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: stain with broad specifi...
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UC San Diego Library Digital Collections
2021
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Online Access: | https://dx.doi.org/10.6075/j0gh9j4s https://library.ucsd.edu/dc/object/bb9188821b |
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ftdatacite:10.6075/j0gh9j4s 2024-02-04T09:54:39+01:00 CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... Moisan, Tiffany Sosinsky, Gina Buitenhuys, Casey Ellisman, Mark 2021 image/jpeg text/plain https://dx.doi.org/10.6075/j0gh9j4s https://library.ucsd.edu/dc/object/bb9188821b unknown UC San Diego Library Digital Collections https://dx.doi.org/10.6075/j0p84bp1 https://dx.doi.org/10.7295/w9cil39986 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Phaeocystis antarctica Chloroplast thylakoid membrane organization algae photosynthetic electron transport chain thylakoid Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0gh9j4s10.6075/j0p84bp110.7295/w9cil3998610.6075/j0r20zxn 2024-01-05T01:23:18Z Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment Relation to intact cell: microtome-sectioned tissue Imaging mode: illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: stain with broad specificity Visualization methods: lead salt; uranyl salt; cationic colloidal gold Processing history: recorded image Data qualification: Processed ... : Culture conditions. Cultures of colonial P. antarctica (CCMP 1374) were grown semi-continuously for 5-8 generations in f/2 medium (Guillard and Ryther 1962) under continuous blue light at 4°C at irradiances of 14 and 259 µmol quanta m-2 s-1. Specific growth rate. Specific growth rate was estimated by a linear regression of loge transformed daily determinations of in vivo fluorescence intensity (n=2) measured with a Turner Model 10 fluorometer. Sample preparation for electron microscopy. P. antarctica colonies were fixed on ice with a 2% glutaraldehyde and 1.3% osmium tetroxide solution for 30 minutes and rinsed in distilled water. Cells were dehydrated through a series of ethanol: water washes (25:75, 50:50, 75:25, 95:5), three 100% ethanol washes and finally through three washes of 100% acetone. Cells were pelleted and fixed in an Epon resin. The fixation process lends itself to a breakup of the colonial matrix and we were able to examine P. antarctica individual colonial cells using electron tomography. ... Dataset Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
unknown |
topic |
Phaeocystis antarctica Chloroplast thylakoid membrane organization algae photosynthetic electron transport chain thylakoid |
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Phaeocystis antarctica Chloroplast thylakoid membrane organization algae photosynthetic electron transport chain thylakoid Moisan, Tiffany Sosinsky, Gina Buitenhuys, Casey Ellisman, Mark CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
topic_facet |
Phaeocystis antarctica Chloroplast thylakoid membrane organization algae photosynthetic electron transport chain thylakoid |
description |
Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment Relation to intact cell: microtome-sectioned tissue Imaging mode: illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: stain with broad specificity Visualization methods: lead salt; uranyl salt; cationic colloidal gold Processing history: recorded image Data qualification: Processed ... : Culture conditions. Cultures of colonial P. antarctica (CCMP 1374) were grown semi-continuously for 5-8 generations in f/2 medium (Guillard and Ryther 1962) under continuous blue light at 4°C at irradiances of 14 and 259 µmol quanta m-2 s-1. Specific growth rate. Specific growth rate was estimated by a linear regression of loge transformed daily determinations of in vivo fluorescence intensity (n=2) measured with a Turner Model 10 fluorometer. Sample preparation for electron microscopy. P. antarctica colonies were fixed on ice with a 2% glutaraldehyde and 1.3% osmium tetroxide solution for 30 minutes and rinsed in distilled water. Cells were dehydrated through a series of ethanol: water washes (25:75, 50:50, 75:25, 95:5), three 100% ethanol washes and finally through three washes of 100% acetone. Cells were pelleted and fixed in an Epon resin. The fixation process lends itself to a breakup of the colonial matrix and we were able to examine P. antarctica individual colonial cells using electron tomography. ... |
format |
Dataset |
author |
Moisan, Tiffany Sosinsky, Gina Buitenhuys, Casey Ellisman, Mark |
author_facet |
Moisan, Tiffany Sosinsky, Gina Buitenhuys, Casey Ellisman, Mark |
author_sort |
Moisan, Tiffany |
title |
CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
title_short |
CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
title_full |
CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
title_fullStr |
CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
title_full_unstemmed |
CIL:39986, Phaeocystis antarctica, algae. In Cell Image Library ... |
title_sort |
cil:39986, phaeocystis antarctica, algae. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0gh9j4s https://library.ucsd.edu/dc/object/bb9188821b |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
https://dx.doi.org/10.6075/j0p84bp1 https://dx.doi.org/10.7295/w9cil39986 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6075/j0gh9j4s10.6075/j0p84bp110.7295/w9cil3998610.6075/j0r20zxn |
_version_ |
1789974612823506944 |