CIL:25307, Drosophila melanogaster. In Cell Image Library ...

• Main Authors: Maresca, Thomas, Salmon, Edward
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: Drosophila melanogaster; spindle; mitosis; S2; Orca
• Online Access: https://dx.doi.org/10.6075/j0g15zrg; https://library.ucsd.edu/dc/object/bb5058461r

Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: differences in adsorption or binding of stain Visualization methods: Green fluorescent proteins from Aequorea Processing history: unprocessed raw data ... : Movie showing kinetochore alignment during mitosis in Drosophila S2 cells expressing GFP-Ndc80 to label kinetochores. One pair of chromatids becomes detached from the spindle, and anaphase is delayed until the pair is recaptured. Images were recorded at 60 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Maresca and Salmon, 2009 J Cell Biol 184:373-281. ...