CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ...
Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD...
Main Authors: | , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
UC San Diego Library Digital Collections
2021
|
Subjects: | |
Online Access: | https://dx.doi.org/10.6075/j0fb51m9 https://library.ucsd.edu/dc/object/bb69692434 |
id |
ftdatacite:10.6075/j0fb51m9 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.6075/j0fb51m9 2024-02-04T10:03:42+01:00 CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... Maddox, Paul S. Hyndman, Francie Monen, Joost Oegema, Karen Desai, Arshad 2021 application/zip image/jpeg video/mp4 text/plain https://dx.doi.org/10.6075/j0fb51m9 https://library.ucsd.edu/dc/object/bb69692434 unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil28780 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution Non Commercial Share Alike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode cc-by-nc-sa-4.0 kinetochore Caenorhabditis elegans mitosis metaphase CeCENP-A prophase nuclear chromosome embryo Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0fb51m910.7295/w9cil2878010.6075/j0r20zxn 2024-01-05T01:28:39Z Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification. ... : Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in strain OD101. During prophase, discrete foci of GFP signal within the nucleus coalesce to form holocentric kinetochore plates. After nuclear envelope breakdown, chromosomes congress to the metaphase plate and are seen as paired lines. At metaphase, sister kinetochores form two lines facing opposite poles. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right. Images were acquired at 10-s intervals. ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
kinetochore Caenorhabditis elegans mitosis metaphase CeCENP-A prophase nuclear chromosome embryo |
spellingShingle |
kinetochore Caenorhabditis elegans mitosis metaphase CeCENP-A prophase nuclear chromosome embryo Maddox, Paul S. Hyndman, Francie Monen, Joost Oegema, Karen Desai, Arshad CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
topic_facet |
kinetochore Caenorhabditis elegans mitosis metaphase CeCENP-A prophase nuclear chromosome embryo |
description |
Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification. ... : Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-CeCENP-A in strain OD101. During prophase, discrete foci of GFP signal within the nucleus coalesce to form holocentric kinetochore plates. After nuclear envelope breakdown, chromosomes congress to the metaphase plate and are seen as paired lines. At metaphase, sister kinetochores form two lines facing opposite poles. The video is ∼12 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the left, and GFP is shown on the right. Images were acquired at 10-s intervals. ... |
format |
Dataset |
author |
Maddox, Paul S. Hyndman, Francie Monen, Joost Oegema, Karen Desai, Arshad |
author_facet |
Maddox, Paul S. Hyndman, Francie Monen, Joost Oegema, Karen Desai, Arshad |
author_sort |
Maddox, Paul S. |
title |
CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
title_short |
CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
title_full |
CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
title_fullStr |
CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
title_full_unstemmed |
CIL:28780, Caenorhabditis elegans, embryo. In Cell Image Library ... |
title_sort |
cil:28780, caenorhabditis elegans, embryo. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0fb51m9 https://library.ucsd.edu/dc/object/bb69692434 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil28780 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution Non Commercial Share Alike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode cc-by-nc-sa-4.0 |
op_doi |
https://doi.org/10.6075/j0fb51m910.7295/w9cil2878010.6075/j0r20zxn |
_version_ |
1789971300270211072 |