CIL:27831, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

• Main Authors: Uetake, Yumi, Sluder, Greenfield
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: cell motility; cell morphology; Homo sapiens; retinal pigment epithelium; hTERT-RPE1; Orca
• Online Access: https://dx.doi.org/10.6075/j0d799cn; https://library.ucsd.edu/dc/object/bb4273485v

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software. ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: phase contrast microscopy Parameter imaged: elastic scattering of photons Source of contrast: differences in amount of elastic light scattering Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ...