CIL: 50728, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library ...

• Main Authors: Ivanchenko, Maryna V., Cicconet, Marcelo, Jandal, Hoor Al, Wu, Xudong, Corey, David P., Indzhykulian, Artur A.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: Hair cell stereocilia; mitochondria; Mus musculus; Inner ear outer hair cell; Native auditory hair cells at postnatal day 5; Polycystic Kidney and Hepatic Disease 1-Like 1 PKHD1L1 labeling gold beads; Cuticular plate; Helios; Orca
• Online Access: https://dx.doi.org/10.6075/j0cr5ss5; https://library.ucsd.edu/dc/object/bb6835576b

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ...