CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ...
Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Ra...
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UC San Diego Library Digital Collections
2021
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Online Access: | https://dx.doi.org/10.6075/j0c53jmd https://library.ucsd.edu/dc/object/bb2498057j |
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ftdatacite:10.6075/j0c53jmd 2024-02-04T10:03:42+01:00 CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... Wittmann, Torsten Bokoch, Gary M. Waterman-Storer, Clare M. 2021 video/mp4 application/zip image/jpeg text/plain https://dx.doi.org/10.6075/j0c53jmd https://library.ucsd.edu/dc/object/bb2498057j unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil11836 https://dx.doi.org/10.1083/jcb.200303082 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution Non Commercial Share Alike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode cc-by-nc-sa-4.0 Pt K1 NBL-3 microtubule Potorous tridactylus microtubule polymerization active Rac epithelial cell Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0c53jmd10.7295/w9cil1183610.1083/jcb.20030308210.6075/j0r20zxn 2024-01-05T01:25:55Z Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(Q61L) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 2 of associated paper (J Cell Biol, 161:845-851, 2003). ... : Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Pt K1 NBL-3 microtubule Potorous tridactylus microtubule polymerization active Rac epithelial cell |
spellingShingle |
Pt K1 NBL-3 microtubule Potorous tridactylus microtubule polymerization active Rac epithelial cell Wittmann, Torsten Bokoch, Gary M. Waterman-Storer, Clare M. CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
topic_facet |
Pt K1 NBL-3 microtubule Potorous tridactylus microtubule polymerization active Rac epithelial cell |
description |
Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(Q61L) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 2 of associated paper (J Cell Biol, 161:845-851, 2003). ... : Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... |
format |
Dataset |
author |
Wittmann, Torsten Bokoch, Gary M. Waterman-Storer, Clare M. |
author_facet |
Wittmann, Torsten Bokoch, Gary M. Waterman-Storer, Clare M. |
author_sort |
Wittmann, Torsten |
title |
CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
title_short |
CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
title_full |
CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
title_fullStr |
CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ... |
title_sort |
cil:11836, potorous tridactylus, epithelial cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0c53jmd https://library.ucsd.edu/dc/object/bb2498057j |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil11836 https://dx.doi.org/10.1083/jcb.200303082 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution Non Commercial Share Alike 4.0 International https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode cc-by-nc-sa-4.0 |
op_doi |
https://doi.org/10.6075/j0c53jmd10.7295/w9cil1183610.1083/jcb.20030308210.6075/j0r20zxn |
_version_ |
1789971301802180608 |