CIL:11836, Potorous tridactylus, epithelial cell. In Cell Image Library ...

• Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: Pt K1 NBL-3; microtubule; Potorous tridactylus; microtubule polymerization; active Rac; epithelial cell; Orca
• Online Access: https://dx.doi.org/10.6075/j0c53jmd; https://library.ucsd.edu/dc/object/bb2498057j

Microtubules in a constitutively active Rac1(Q61L)-expressing PtK1 cell. Microtubules are subjected to continuous retrograde flow away from the cell edge, but many plus ends undergo net growth and remain close to the edge. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(Q61L) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 2 of associated paper (J Cell Biol, 161:845-851, 2003). ... : Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ...