CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ...

• Main Authors: Deshmukh, M., Dimitru, R., Gama, V., Fagan, B.M., Bower, J.J., Swahari, V., Pevney, L.H.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: Golgi apparatus; apoptosis; nucleus; embryonic stem cell; Homo sapiens; Triton; Bower; Orca
• Online Access: https://dx.doi.org/10.6075/j0bv7fzr; https://library.ucsd.edu/dc/object/bb30450517

Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope. See also: Dumitru R, Gama V, Fagan BM, Bower JJ, Swahari V, Pevny LH, Deshmukh M. 2012. Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis. Mol Cell. 46(5):573-83. ... : Preparation: formaldehyde fixed tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; differences in adsorption or binding of stain Visualization methods: Hoechst 33258; Alexa Fluor 488 Data qualification: Processed ...