CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ...
Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope. See...
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2021
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Online Access: | https://dx.doi.org/10.6075/j0bv7fzr https://library.ucsd.edu/dc/object/bb30450517 |
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ftdatacite:10.6075/j0bv7fzr 2024-02-04T10:03:42+01:00 CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... Deshmukh, M. Dimitru, R. Gama, V. Fagan, B.M. Bower, J.J. Swahari, V. Pevney, L.H. 2021 application/zip image/jpeg text/plain https://dx.doi.org/10.6075/j0bv7fzr https://library.ucsd.edu/dc/object/bb30450517 unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil42451 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 Golgi apparatus apoptosis nucleus embryonic stem cell Homo sapiens Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j0bv7fzr10.7295/w9cil4245110.6075/j0r20zxn 2024-01-05T01:15:57Z Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope. See also: Dumitru R, Gama V, Fagan BM, Bower JJ, Swahari V, Pevny LH, Deshmukh M. 2012. Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis. Mol Cell. 46(5):573-83. ... : Preparation: formaldehyde fixed tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; differences in adsorption or binding of stain Visualization methods: Hoechst 33258; Alexa Fluor 488 Data qualification: Processed ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) Triton ENVELOPE(-55.615,-55.615,49.517,49.517) Bower ENVELOPE(160.500,160.500,-72.617,-72.617) |
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DataCite Metadata Store (German National Library of Science and Technology) |
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topic |
Golgi apparatus apoptosis nucleus embryonic stem cell Homo sapiens |
spellingShingle |
Golgi apparatus apoptosis nucleus embryonic stem cell Homo sapiens Deshmukh, M. Dimitru, R. Gama, V. Fagan, B.M. Bower, J.J. Swahari, V. Pevney, L.H. CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
topic_facet |
Golgi apparatus apoptosis nucleus embryonic stem cell Homo sapiens |
description |
Cells were fixed with formaldehyde, permeabilized using Triton X-100, and stained with anti-Bax antibody 6A7 which is specific to the active form (red) and Hoechst dye to stain nuclei (red). Images were recorded on a Hamamatsu ORCA-ER digital camera mounted on a Leica DMIRE2 inverted microscope. See also: Dumitru R, Gama V, Fagan BM, Bower JJ, Swahari V, Pevny LH, Deshmukh M. 2012. Human embryonic stem cells have constitutively active Bax at the Golgi and are primed to undergo rapid apoptosis. Mol Cell. 46(5):573-83. ... : Preparation: formaldehyde fixed tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of epitope; differences in adsorption or binding of stain Visualization methods: Hoechst 33258; Alexa Fluor 488 Data qualification: Processed ... |
format |
Dataset |
author |
Deshmukh, M. Dimitru, R. Gama, V. Fagan, B.M. Bower, J.J. Swahari, V. Pevney, L.H. |
author_facet |
Deshmukh, M. Dimitru, R. Gama, V. Fagan, B.M. Bower, J.J. Swahari, V. Pevney, L.H. |
author_sort |
Deshmukh, M. |
title |
CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
title_short |
CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
title_full |
CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
title_fullStr |
CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:42451, Homo sapiens, embryonic stem cell. In Cell Image Library ... |
title_sort |
cil:42451, homo sapiens, embryonic stem cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j0bv7fzr https://library.ucsd.edu/dc/object/bb30450517 |
long_lat |
ENVELOPE(-55.615,-55.615,49.517,49.517) ENVELOPE(160.500,160.500,-72.617,-72.617) |
geographic |
Triton Bower |
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Triton Bower |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil42451 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j0bv7fzr10.7295/w9cil4245110.6075/j0r20zxn |
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1789971289315737600 |