CIL: 50754, Drosophila melanogaster. In Cell Image Library ...

• Main Authors: Endow, Sharyn A., Miller, Sara E., Thao Ly, Phuong
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: Drosophila melanogaster; L1 larval brain; Orca
• Online Access: https://dx.doi.org/10.6075/j08w3cq0; https://library.ucsd.edu/dc/object/bb1511297g

Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : Specimen preparation: Larval brains (LBs) were prepared from starved (amino acid-restricted) first instar (L1) larvae to enrich for quiescent neural stem cells. The LBs were glutaraldehyde-fixed, tannic acid and OsO4-stained, embedded in resin, thin-sectioned (60-70 nm) and stained with uranyl acetate/lead citrate, then observed by TEM. ...