CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...
Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferre...
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2021
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Online Access: | https://dx.doi.org/10.6075/j08c9vdv https://library.ucsd.edu/dc/object/bb6048189f |
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ftdatacite:10.6075/j08c9vdv 2024-02-04T10:04:09+01:00 CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... Ladinsky, Mark Howell, Kathryn E. 2021 application/zip image/jpeg text/plain https://dx.doi.org/10.6075/j08c9vdv https://library.ucsd.edu/dc/object/bb6048189f unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil12923 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 lipid storage protein binding glandular epithelial cell trans-Golgi network secretion lactation Rattus rattus Mitochondrion lipid particle mammary alveolar cell milk secreting cell smooth endoplasmic reticulum Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j08c9vdv10.7295/w9cil1292310.6075/j0r20zxn 2024-01-05T01:15:57Z Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then ... : Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements;intensitiesquantitation ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
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collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
lipid storage protein binding glandular epithelial cell trans-Golgi network secretion lactation Rattus rattus Mitochondrion lipid particle mammary alveolar cell milk secreting cell smooth endoplasmic reticulum |
spellingShingle |
lipid storage protein binding glandular epithelial cell trans-Golgi network secretion lactation Rattus rattus Mitochondrion lipid particle mammary alveolar cell milk secreting cell smooth endoplasmic reticulum Ladinsky, Mark Howell, Kathryn E. CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
topic_facet |
lipid storage protein binding glandular epithelial cell trans-Golgi network secretion lactation Rattus rattus Mitochondrion lipid particle mammary alveolar cell milk secreting cell smooth endoplasmic reticulum |
description |
Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by sequential incubation with antibodies to adipophilin (guinea pig anti-adipophilin) and TGN38 (mouse monoclonal 2F7) and colloidal gold-conjugated secondary antibodies (15 nm anti-mouse, 10 nm anti-guinea pig; Ted Pella Inc., Redding, CA) and then ... : Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements;intensitiesquantitation ... |
format |
Dataset |
author |
Ladinsky, Mark Howell, Kathryn E. |
author_facet |
Ladinsky, Mark Howell, Kathryn E. |
author_sort |
Ladinsky, Mark |
title |
CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_short |
CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full |
CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_fullStr |
CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:12923, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_sort |
cil:12923, rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j08c9vdv https://library.ucsd.edu/dc/object/bb6048189f |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.7295/w9cil12923 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j08c9vdv10.7295/w9cil1292310.6075/j0r20zxn |
_version_ |
1789972188778987520 |