CIL:12598, Canis lupus familiaris, epithelial cell. In Cell Image Library ...

• Main Authors: Baccei, Anna, Lancelle, Susan
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: focal adhesion; stress fiber; MDCK; epithelial cell; Canis lupus familiaris; Canis lupus
• Online Access: https://dx.doi.org/10.6075/j06w9b4m; https://library.ucsd.edu/dc/object/bb0007566x

Preparation: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; lead citrate Relation to intact cell: dispersed cells in vitro; sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain Visualization methods: uranyl salt Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements ... : Parallel stress fibers on the ventral face of an MDCK cell, just inside the plasma membrane. This transmission electron micrograph of a flat-embedded MDCK cell was taken 60-70nm from the interface between the cell and the coverslip on which it was grown and fixed. The cells were cultured on Thermanox® coverslips, then chemically fixed with glutaraldehyde and osmium tetroxide before being stained en bloc with uranyl acetate. The cells were infiltrated with Spurr's® resin, placed under a vacuum for fifteen minutes, and then polymerized at 70° C. The coverslips were peeled away from the hardened resin wafers, which were then cut and thin-sectioned with a diamond knife. The 65 nm sections were placed on grids and post-stained with uranyl acetate and lead citrate. They were imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV. Dark spots at somewhat regular intervals along the stress fibers are likely to be focal adhesion sites. Scale bar = 1µm. ...