CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 sca...
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2022
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Online Access: | https://dx.doi.org/10.6075/j0668cm1 https://library.ucsd.edu/dc/object/bb9634248d |
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ftdatacite:10.6075/j0668cm1 2024-02-04T10:03:42+01:00 CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... Buckles, Thomas 2022 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0668cm1 https://library.ucsd.edu/dc/object/bb9634248d unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil50962 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Cell polarity establishment and maintenance Mus musculus Whole cell RAW 264.7 TIB-71 Dataset dataset 2022 ftdatacite https://doi.org/10.6075/j0668cm110.7295/w9cil5096210.6075/j0r20zxn 2024-01-05T01:24:16Z Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
unknown |
topic |
Cell polarity establishment and maintenance Mus musculus Whole cell RAW 264.7 TIB-71 |
spellingShingle |
Cell polarity establishment and maintenance Mus musculus Whole cell RAW 264.7 TIB-71 Buckles, Thomas CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
topic_facet |
Cell polarity establishment and maintenance Mus musculus Whole cell RAW 264.7 TIB-71 |
description |
Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : populations of ~1200 cells plated on glass in standard media were imaged at 37º C in each experiment. A target subpopulation of ~100 cells display a readily observed extended, ruffling leading edge pseudopod . The cells exhibiting these deployed, extended pseudopods were counted in each unit area at 6 timepoints during the 3 hr observation period following rapid addition of drug to its standard working concentration. Altogether, 6 experiments were carried out for each treatment, yielding average timecourses summarizing observations of ~600 polarized cells. Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening ... |
format |
Dataset |
author |
Buckles, Thomas |
author_facet |
Buckles, Thomas |
author_sort |
Buckles, Thomas |
title |
CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_short |
CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full |
CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_fullStr |
CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full_unstemmed |
CIL: 50962, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_sort |
cil: 50962, mus musculus, raw 264.7 tib-71. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2022 |
url |
https://dx.doi.org/10.6075/j0668cm1 https://library.ucsd.edu/dc/object/bb9634248d |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil50962 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6075/j0668cm110.7295/w9cil5096210.6075/j0r20zxn |
_version_ |
1789971315292110848 |