CIL:38595, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...

• Main Authors: Ladinsky, Mark, Howell, Kathryn E.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: mammary alveolar cell; lipid storage; lactation; smooth endoplasmic reticulum; glandular epithelial cell; milk secreting cell; Rattus rattus; trans-Golgi network; protein binding; lipid particle; secretion; Mitochondrion
• Online Access: https://dx.doi.org/10.6075/j0639p2x; https://library.ucsd.edu/dc/object/bb6423785m

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‚ÄêGolgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid. ... : Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM); illumination by electrons; detection of electrons Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements;intensitiesquantitation ...