CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ...
Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 sca...
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2022
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Online Access: | https://dx.doi.org/10.6075/j0610zr3 https://library.ucsd.edu/dc/object/bb07946083 |
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ftdatacite:10.6075/j0610zr3 2023-10-01T03:58:44+02:00 CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... Buckles, Thomas 2022 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j0610zr3 https://library.ucsd.edu/dc/object/bb07946083 unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil51867 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 Whole cell Mus musculus RAW 264.7 TIB-71 Cell polarity establishment and maintenance Dataset dataset 2022 ftdatacite https://doi.org/10.6075/j0610zr310.7295/w9cil5186710.6075/j0r20zxn 2023-09-04T14:18:18Z Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK" 5. The 16 bit image will typically look dark and will require ... Dataset Orca DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Whole cell Mus musculus RAW 264.7 TIB-71 Cell polarity establishment and maintenance |
spellingShingle |
Whole cell Mus musculus RAW 264.7 TIB-71 Cell polarity establishment and maintenance Buckles, Thomas CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
topic_facet |
Whole cell Mus musculus RAW 264.7 TIB-71 Cell polarity establishment and maintenance |
description |
Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ... : ~1200 cells were imaged on glass. More gradual additions of drug or carrier were carried out in four equal increments at 0, 30, 60, 90 min, together yielding the same final drug concentration indicated in rapid addition studies. The subpopulation of ~100 cells with extended, leading edge pseudopods at t = 0 were imaged and counted in each unit area at the indicated timepoints during a 125 min observation period T=37C Images are provided as uncorrected, 16 bit .nd2 files to preserve all original data. To view these .nd2 files: 1. Download ImageJ viewer at (https://imagej.nih.gov/ij/download.html) 2. Download the BioFormats.jar package at (https://www.openmicroscopy.org/bio-formats/downloads/) 3. Open the ImageJ directory after installation and drag the extracted BioFormats.jar package into the "Plugins" subdirectory 4. Open an .nd2 image using imageJ and select opening options when prompted, if desired. If no modifications are desired, select "OK" 5. The 16 bit image will typically look dark and will require ... |
format |
Dataset |
author |
Buckles, Thomas |
author_facet |
Buckles, Thomas |
author_sort |
Buckles, Thomas |
title |
CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_short |
CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full |
CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_fullStr |
CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_full_unstemmed |
CIL: 51867, Mus musculus, RAW 264.7 TIB-71. In Cell Image Library ... |
title_sort |
cil: 51867, mus musculus, raw 264.7 tib-71. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2022 |
url |
https://dx.doi.org/10.6075/j0610zr3 https://library.ucsd.edu/dc/object/bb07946083 |
genre |
Orca |
genre_facet |
Orca |
op_relation |
https://dx.doi.org/10.7295/w9cil51867 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Attribution 4.0 International https://creativecommons.org/licenses/by/4.0/legalcode cc-by-4.0 |
op_doi |
https://doi.org/10.6075/j0610zr310.7295/w9cil5186710.6075/j0r20zxn |
_version_ |
1778531837392453632 |