CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...
Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferre...
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2021
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Online Access: | https://dx.doi.org/10.6075/j05h7ff1 https://library.ucsd.edu/dc/object/bb29082279 |
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ftdatacite:10.6075/j05h7ff1 2024-02-04T10:04:09+01:00 CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... Ladinsky, Mark Howell, Kathryn E. 2021 image/jpeg application/zip text/plain https://dx.doi.org/10.6075/j05h7ff1 https://library.ucsd.edu/dc/object/bb29082279 unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil12867 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 trans-Golgi network lactation secretion lipid particle glandular epithelial cell protein binding Mitochondrion milk secreting cell lipid storage mammary alveolar cell smooth endoplasmic reticulum Rattus rattus Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j05h7ff110.7295/w9cil1286710.6075/j0r20zxn 2024-01-05T01:15:57Z Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by incubation with an antibody to TGN38 (2F7, mouse monoclonal) followed by colloidal gold-conjugated secondary antibody (10 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in ... : Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
trans-Golgi network lactation secretion lipid particle glandular epithelial cell protein binding Mitochondrion milk secreting cell lipid storage mammary alveolar cell smooth endoplasmic reticulum Rattus rattus |
spellingShingle |
trans-Golgi network lactation secretion lipid particle glandular epithelial cell protein binding Mitochondrion milk secreting cell lipid storage mammary alveolar cell smooth endoplasmic reticulum Rattus rattus Ladinsky, Mark Howell, Kathryn E. CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
topic_facet |
trans-Golgi network lactation secretion lipid particle glandular epithelial cell protein binding Mitochondrion milk secreting cell lipid storage mammary alveolar cell smooth endoplasmic reticulum Rattus rattus |
description |
Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by incubation with an antibody to TGN38 (2F7, mouse monoclonal) followed by colloidal gold-conjugated secondary antibody (10 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in ... : Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements ... |
format |
Dataset |
author |
Ladinsky, Mark Howell, Kathryn E. |
author_facet |
Ladinsky, Mark Howell, Kathryn E. |
author_sort |
Ladinsky, Mark |
title |
CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_short |
CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full |
CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_fullStr |
CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:12867, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_sort |
cil:12867, rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j05h7ff1 https://library.ucsd.edu/dc/object/bb29082279 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.7295/w9cil12867 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j05h7ff110.7295/w9cil1286710.6075/j0r20zxn |
_version_ |
1789972179054493696 |