CIL:35278, Rattus rattus, hepatocyte. In Cell Image Library ...

• Main Authors: Cammer, Michael, Novikoff, Phyllis
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: hepatocyte; cytoplasmic microtubule; microtubule organizing center; interphase; TS25,liver; Rattus rattus
• Online Access: https://dx.doi.org/10.6075/j05h7f6w; https://library.ucsd.edu/dc/object/bb7891276x

Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: Rhodamine; primary antibody plus labeled secondary antibody Processing history: 3D-deconvolution Data qualification: Processed;spatialmeasurements ... : Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus. This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists. ...