CIL:35278, Rattus rattus, hepatocyte. In Cell Image Library ...
Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods:...
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Online Access: | https://dx.doi.org/10.6075/j05h7f6w https://library.ucsd.edu/dc/object/bb7891276x |
| Summary: | Preparation: formaldehyde fixed tissue; glutaraldehyde fixed tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: Rhodamine; primary antibody plus labeled secondary antibody Processing history: 3D-deconvolution Data qualification: Processed;spatialmeasurements ... : Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus. This is a single optical section a few microns up from the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists. ... |
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