CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ...
Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in...
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2021
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Online Access: | https://dx.doi.org/10.6075/j04x56zx https://library.ucsd.edu/dc/object/bb0450866n |
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ftdatacite:10.6075/j04x56zx 2024-02-04T10:04:09+01:00 CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... Ladinsky, Mark Howell, Kathryn E. 2021 application/zip image/jpeg text/plain https://dx.doi.org/10.6075/j04x56zx https://library.ucsd.edu/dc/object/bb0450866n unknown UC San Diego Library Digital Collections https://dx.doi.org/10.7295/w9cil12852 https://dx.doi.org/10.6075/j0r20zxn Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 protein binding lipid storage lactation smooth endoplasmic reticulum secretion Mitochondrion mammary alveolar cell milk secreting cell Golgi medial cisterna Rattus rattus lipid particle glandular epithelial cell Dataset dataset 2021 ftdatacite https://doi.org/10.6075/j04x56zx10.7295/w9cil1285210.6075/j0r20zxn 2024-01-05T01:30:46Z Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by incubation with an antibody to MG-160 (mouse monoclonal) followed by colloidal gold-conjugated secondary antibody (10 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in ... Dataset Rattus rattus DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
protein binding lipid storage lactation smooth endoplasmic reticulum secretion Mitochondrion mammary alveolar cell milk secreting cell Golgi medial cisterna Rattus rattus lipid particle glandular epithelial cell |
spellingShingle |
protein binding lipid storage lactation smooth endoplasmic reticulum secretion Mitochondrion mammary alveolar cell milk secreting cell Golgi medial cisterna Rattus rattus lipid particle glandular epithelial cell Ladinsky, Mark Howell, Kathryn E. CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
topic_facet |
protein binding lipid storage lactation smooth endoplasmic reticulum secretion Mitochondrion mammary alveolar cell milk secreting cell Golgi medial cisterna Rattus rattus lipid particle glandular epithelial cell |
description |
Preparation: cryostat-sectioned tissue; formaldehyde fixed tissue; embedded tissue; methylcellulose embedded Relation to intact cell: sectioned tissue Item type: recorded image Imaging mode: transmission electron microscopy (TEM) Parameter imaged: electron density Source of contrast: differences in adsorption or binding of stain; distribution of a specific protein Visualization methods: gold; uranyl salt; primary antibody plus labeled secondary antibody Processing history: unprocessed raw data Data qualification: Raw;spatialmeasurements ... : Tissue was processed for immunoelectron microscopy using a modified Tokuyasu method. Briefly, minced tissue was fixed in 4% paraformaldehyde containing 5% sucrose and 100 mM HEPES and infiltrated with PBS containing 2.1 M sucrose over 10 h, with repeated solution changes. Fixed tissue was transferred to an aluminum cryosectioning stub (Ted Pella, Inc., Redding, CA) and immediately frozen in liquid nitrogen. Semithin (90 nm) cryosections were cut at -110C with an UltraCut UCT/FCS cryomicrotome (Leica), using a diamond knife (Diatome) and transferred to a Formvar-coated, carbon-coated, glow-discharged 100-mesh copper-rhodium electron microscopy grid. Following blocking of nonspecific antibody binding sites with 10% calf serum in PBS, the sections were labeled by incubation with an antibody to MG-160 (mouse monoclonal) followed by colloidal gold-conjugated secondary antibody (10 nm anti-mouse; Ted Pella Inc., Redding, CA) and then negatively stained and embedded with 1% uranyl acetate, 1% methylcellulose in ... |
format |
Dataset |
author |
Ladinsky, Mark Howell, Kathryn E. |
author_facet |
Ladinsky, Mark Howell, Kathryn E. |
author_sort |
Ladinsky, Mark |
title |
CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_short |
CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full |
CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_fullStr |
CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_full_unstemmed |
CIL:12852, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library ... |
title_sort |
cil:12852, rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. in cell image library ... |
publisher |
UC San Diego Library Digital Collections |
publishDate |
2021 |
url |
https://dx.doi.org/10.6075/j04x56zx https://library.ucsd.edu/dc/object/bb0450866n |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_relation |
https://dx.doi.org/10.7295/w9cil12852 https://dx.doi.org/10.6075/j0r20zxn |
op_rights |
Creative Commons Zero v1.0 Universal https://creativecommons.org/publicdomain/zero/1.0/legalcode cc0-1.0 |
op_doi |
https://doi.org/10.6075/j04x56zx10.7295/w9cil1285210.6075/j0r20zxn |
_version_ |
1789972177562370048 |