CIL: 50698, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library ...

• Main Authors: Ivanchenko, Maryna V., Cicconet, Marcelo, Jandal, Hoor Al, Wu, Xudong, Corey, David P., Indzhykulian, Artur A.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2022
• Subjects: Hair cell stereocilia; Cuticular plate; mitochondria; Mus musculus; Native auditory Hair Cells at postnatal day 4; Inner ear outer hair cell; Polycystic Kidney and Hepatic Disease 1-Like 1 PKHD1L1 labeling gold beads; Helios; Orca
• Online Access: https://dx.doi.org/10.6075/j04f1q3k; https://library.ucsd.edu/dc/object/bb4275722k

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample. 3.2_Cell_3_Stack_Cropped_Aligned is a multipage TIFF file containing aligned images of Cell#3 (2.6×2.6×15 nm³ voxel size). Contains only the relevant volume of Cell#3, and was cropped to reduce the file size. This file is a part of Dataset 3, comprised of files related to the Cell #3 of current study. The numbering order of the files reflects the order of operations performed during the analysis. ... : Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective ...