CIL:10105, Homo sapiens, epithelial cell, retinal pigmented epithelial cell. In Cell Image Library ...

• Main Authors: Mettlen, Marcel, Schmid, Sandra L.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: retinal pigmented epithelial cell; nucleus; clathrin coat of coated pit; clathrin light chain a; Homo sapiens; epithelial cell; focal adhesion; Lambda; Olympus; Triton; Orca
• Online Access: https://dx.doi.org/10.6075/j04b30dn; https://library.ucsd.edu/dc/object/bb7379218w

Human retinal pigmented epithelial (RPE) cells labeled for clathrin-coated pits (green), focal adhesions (red) and nuclei (blue). RPE cells stabily expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) were plated directly on glass coverslides. After 72 hrs, cells were fixed for 2 min in a 1:1 mixture of 2% paraformaldehyde/0.5% Triton X-100, followed by a 30 min fixation in 4% paraformaldehyde. Cells were then stained with mouse monoclonal antibodies to paxillin, detected with goat anti-mouse Alexa 568. Nuclei were stained with DAPI. Image was collected on an Olympus IX71 inverted microscope coupled to a Hamamatsu ORCA-ER digital camera using a 60x 1.42 NA Plan Apo N objective (Olympus). The exposure times were 200, 500 and 70 msec for the red, green and blue channel, respectively. A xenon-arc lamp (Lambda LS stand-alone illuminator; Sutter Instrument) was used with adequat filters (Chroma). Bar: 5µm ... : Preparation: formaldehyde fixed tissue; detergent permeabilized Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: EGFP; Alexa Fluor 568; 4',6-diamidino-2-phenylindole (DAPI); primary antibody plus labeled secondary antibody Processing history: 3 color combine ...