CIL:34849, Homo sapiens, retinal pigment epithelium. In Cell Image Library ...

• Main Authors: Hatano, Toshiyuki, Sluder, Greenfield
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: mitosis; retinal pigment epithelium; cell proliferation; Homo sapiens; hTERT-RPE1; Orca
• Online Access: https://dx.doi.org/10.6075/j02v2f18; https://library.ucsd.edu/dc/object/bb7720619z

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 100x HCX PLAN APO NA 1.35 DIC objective lens. images were captured at 5 sec intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software. ... : Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ...