CIL:44508, Canis lupus familiaris, kidney epithelial cell. In Cell Image Library ...

• Main Authors: Zaritsky, Assaf, Kaplan, Doron
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: cell migration; MDCK-II; Canis lupus familiaris; kidney epithelial cell; wound healing, spreading of epidermal cells; Darby; Canis lupus
• Online Access: https://dx.doi.org/10.6075/j02r3r2s; https://library.ucsd.edu/dc/object/bb7652602g

Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: recorded image Imaging mode: differential interference contrast microscopy Parameter imaged: optical path length gradient Source of contrast: boundaries between regions with different refractive index Visualization methods: visualization of contiguous regions Processing history: unprocessed raw data ... : Time series light microscopy images illustrating a wound healing assay. A monolayer of MDCK (Madin-Darby Canine Kidney) epithelial cells is scratched to create a 'wound' about 300 micrometers in width, after which the growth of the cells to fill the wound is monitored by recording images over the course of 15h.This image is part of a group of 12 that includes control cells (CIL:44501, 502, 506, 507, 508)and after addition of Hepatocyte Growth Factor /Scatter Factor (HGF/SF) to activate HGF/SF-Met signaling (CIL:44503, 504, 505, 509, 510). Also in the group are two movies, one control (CIL:44511), and one HGF/SF treated (CIL:44512). For additional wound healing assays see CIL:43031 and images grouped with it. ...