CIL:39976, Phaeocystis antarctica, algae. In Cell Image Library ...
Single tilt image taken at zero degrees tilt of a 0.75 um section of blue green algae imaged using intermediate voltage electron microscopy. Contrast is reversed so that electron dense structures appear bright. This image has been downsampled from the raw data image which can be accessed using the l...
| Main Authors: | , , , |
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| Format: | Dataset |
| Language: | unknown |
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UC San Diego Library Digital Collections
2021
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| Subjects: | |
| Online Access: | https://dx.doi.org/10.6075/j01c1x03 https://library.ucsd.edu/dc/object/bb2635858t |
| Summary: | Single tilt image taken at zero degrees tilt of a 0.75 um section of blue green algae imaged using intermediate voltage electron microscopy. Contrast is reversed so that electron dense structures appear bright. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Moisan, T., Ellisman, M. H., Buitenhuys, C.W., Sosinsky, G. E., (2006) Differences in Chloroplast Ultrastructure of Phaeocystis antarctica in High and Low Light Conditions, Marine Biology, 149 (6) 1281-1290. ... : Culture conditions. Cultures of colonial P. antarctica (CCMP 1374) were grown semi-continuously for 5-8 generations in f/2 medium (Guillard and Ryther 1962) under continuous blue light at 4°C at irradiances of 14 and 259 µmol quanta m-2 s-1. Specific growth rate. Specific growth rate was estimated by a linear regression of loge transformed daily determinations of in vivo fluorescence intensity (n=2) measured with a Turner Model 10 fluorometer. Sample preparation for electron microscopy. P. antarctica colonies were fixed on ice with a 2% glutaraldehyde and 1.3% osmium tetroxide solution for 30 minutes and rinsed in distilled water. Cells were dehydrated through a series of ethanol: water washes (25:75, 50:50, 75:25, 95:5), three 100% ethanol washes and finally through three washes of 100% acetone. Cells were pelleted and fixed in an Epon resin. The fixation process lends itself to a breakup of the colonial matrix and we were able to examine P. antarctica individual colonial cells using electron tomography. ... |
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