CIL:25304, Drosophila melanogaster. In Cell Image Library ...

• Main Authors: Maresca, Thomas, Salmon, Edward
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: S2; spindle; Drosophila melanogaster; mitosis; Orca
• Online Access: https://dx.doi.org/10.6075/j0125rdt; https://library.ucsd.edu/dc/object/bb64231010

Preparation: living tissue Relation to intact cell: whole mounted tissue Item type: charge coupled device (CCD) Imaging mode: spinning disk confocal microscopy Parameter imaged: fluorescence emission Source of contrast: differences in adsorption or binding of stain Visualization methods: Green fluorescent proteins from Aequorea Processing history: unprocessed raw data ... : Confocal fluorescence time-lapse imaging of a mitotic Drosophila S2 cell expressing GFP-tubulin and treated with 100 nM taxol for 1 h before imaging to induce assembly of monopolar spindles. Images were recorded at 120 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Supplemental Video 5 in Maresca and Salmon, 2009 J Cell Biol 184:373-281. ...