CIL:11837, Potorous tridactylus, epithelial cell. In Cell Image Library ...

• Main Authors: Wittmann, Torsten, Bokoch, Gary M., Waterman-Storer, Clare M.
• Format: Dataset
• Language: unknown
• Published: UC San Diego Library Digital Collections 2021
• Subjects: microtubule polymerization; epithelial cell; dominant-negative Rac; microtubule; Pt K1 NBL-3; Potorous tridactylus; Orca
• Online Access: https://dx.doi.org/10.6075/j00z722g; https://library.ucsd.edu/dc/object/bb37267362

Preparation: living tissue Relation to intact cell: dispersed cells in vitro Item type: recorded image Imaging mode: fluorescence microscopy Parameter imaged: fluorescence emission Source of contrast: distribution of a specific protein Visualization methods: X-Rhodamine Processing history: unprocessed raw data Data qualification: Raw;intensitiesquantitation ... : Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net change in length over time. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(T17N) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 3 of associated paper (J Cell Biol, 161:845-851, 2003). ...