Chlorophyll and phaeopigments from water column samples, collected at selected depths aboard Palmer LTER annual cruises off the coast of the Western Antarctic Peninsula, 1991 – present.

• Main Authors: LTER, Palmer Station Antarctica, Schofield, Oscar, Vernet, Maria, Smith, Raymond
• Format: Dataset
• Language: English
• Published: Environmental Data Initiative 2020
• Subjects: Antarctic; Antarctic Peninsula; Palmer Station; Palmer-Station; Antarc*
• Online Access: https://dx.doi.org/10.6073/pasta/1547495dd8f469c469e3a9fc9cdb6387; https://portal.edirepository.org/nis/mapbrowse?packageid=knb-lter-pal.24.8

Phytoplankton chlorophyll sampling was led by Smith from 1991-2002, and then by Vernet from 2003-2008. Schofield is the third, and current lead, beginning in 2009. Methods have been kept consistent as much as possible over the full time series and different Principal Investigators. Chlorophyll a (Chl a) is the principal photosynthetic pigment of phytoplankton, and is used as a proxy measurement for estimating phytoplankton biomass in water samples. Chl a concentrations reflect the distribution of active phytoplankton spatially and with depth in the water column and their changes over time. Phaeopigments are non-photosynthetic pigments that are degradation products of phytoplankton chlorophylls which form during and after phytoplankton blooms. Water samples are collected throughout the water column along the Western Antarctic Peninsula at regular LTER grid stations where CTD casts are preformed and in surface waters at underway stations, where CTD casts are not done, using the ship’s flow-through seawater system. Water samples are filtered onto GF/F filters, and filters kept frozen at -80°C until analysis at Palmer Station following the completion of the cruise. Fluorometric chlorophyll and phaeopigment analysis is conducted at Palmer Station through acetone extraction of the GF/F filters and measurement of the extract on a Turner 10AU Fluorometer. The primary source of error for phaeopigment measurement is Chlorophyll b. If high amounts of Chlorophyll b are present in the sample, phaeopigments may be overestimated.