Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions ... : تأثير أنيون الفوسفات على استقرار الإنزيمات الجامدة. تأثير طبيعة الإنزيم وبروتوكول التثبيت وظروف التعطيل ...

• Main Authors: Jakub F. Kornecki, Diego Carballares, Roberto Morellon‐Sterling, El-Hocine Siar, Saeid Kashefi, Chafiaa Mazri, Sara Arana‐Peña, Nathália Saraiva Rios, Luciana Rocha Barros Gonçalves, Roberto Fernández‐Lafuente
• Format: Text
• Language: English
• Published: OpenAlex 2020
• Subjects: Enzyme Immobilization Techniques; Molecular Biology; Biochemistry, Genetics and Molecular Biology; Life Sciences; Electrochemical Biosensor Technology; Electrical and Electronic Engineering; FOS Electrical engineering, electronic engineering, information engineering; Engineering; Physical Sciences; Protein Binding Specificity in Human Serum Albumin; Enzyme Stability; Chemistry; Aspergillus oryzae; Enzyme; Immobilized enzyme; Lipase; Phosphate; Candida antarctica; Glutaraldehyde; Chromatography; Agarose; Biochemistry; Rugosa; Antarc*; Antarctica
• Online Access: https://dx.doi.org/10.60692/pb7jy-7v354; https://gresis.osc.int//doi/10.60692/pb7jy-7v354

A destabilizing effect at pH 7 of sodium phosphate on several lipases immobilized via interfacial activation is shown in this work. This paper investigates if this destabilizing effect is extended to other inactivation conditions, immobilization protocols or even other immobilized enzymes (ficin, tr...