Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory)
Protist samples were taken using a 6.2 L Kemmerer sampler, transferred to acid-washed, opaque 20 L plastic containers and stored in the dark at 4°C until processing. They were preserved with paraformaldehyde (0.1% final concentration) and glutaraldehyde (1% final concentration) in duplicate 50 mL po...
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Canadian Cryospheric Information Network
2012
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ftdatacite:10.5443/10927 2023-05-15T15:15:26+02:00 Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) Vincent, Warwick Charvet, Sophie Veillette, Julie 2012 https://dx.doi.org/10.5443/10927 https://www.polardata.ca/pdcsearch/?doi_id=10927 en eng Canadian Cryospheric Information Network Public Abundance Diversity Ellesmere Island Lake A Phytoplankton Protists Taxonomy Water column ArcticNet Network of Centres of Excellence of Canada International Polar Year-Microbial Biodiversity of High Arctic Ecosystems dataset Dataset 2012 ftdatacite https://doi.org/10.5443/10927 2021-11-05T12:55:41Z Protist samples were taken using a 6.2 L Kemmerer sampler, transferred to acid-washed, opaque 20 L plastic containers and stored in the dark at 4°C until processing. They were preserved with paraformaldehyde (0.1% final concentration) and glutaraldehyde (1% final concentration) in duplicate 50 mL polypropylene centrifuge tubes and stored at 4°C for up to 6 months in the dark until analysis; cellular features were well preserved and showed no evidence of degradation during storage. Protists were counted and identified using a combined system of fluorescence, Nomarski interference and Utermöhl sedimentation (FNU). 60 mL samples (16 mL for Lake A at 5 m on 24 August 2008, Lake B at 2 m on 24 August 2008 and Milne Fiord at 5 m on 13 July 2007) were concentrated in Utermöhl sedimentation chambers for 24 hours and stained with DAPI (0.1 mg/mL). Counts and identifications were made with a Zeiss Axiovert 100 inverted epifluorescence microscope under 400 and 1000X magnification (cells > 2 um in diameter). Cells were identified to genus wherever possible and were classified as heterotrophic when no chloroplast was observed. The microscopy detection limit was 2.67 x 10^3 cells/L. A minimum of 400 cells and 15 fields were counted wherever possible. : Purpose: This dataset present phytoplankton and other protist enumerations of Lake A, Lake B, Lake C1, Lake C2, Ward Hunt Lake and Milne Fiord at several depths in 2007 and 2008. The objective of this data was to evaluate the abundance and community composition of the phytoplankton by microscopy. This dataset is also useful to assess the implications for ecosystem structure and function of the increasingly observed loss of perennial lake ice. : Summary: Not Applicable Dataset Arctic ArcticNet Ellesmere Island International Polar Year Milne Fiord Phytoplankton Ward Hunt Island DataCite Metadata Store (German National Library of Science and Technology) Arctic Ellesmere Island Canada Ward Hunt Island ENVELOPE(-74.161,-74.161,83.102,83.102) Milne Fiord ENVELOPE(-81.447,-81.447,82.636,82.636) Island Lake ENVELOPE(-125.856,-125.856,53.733,53.733) Hunt Island ENVELOPE(-100.601,-100.601,58.788,58.788) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Abundance Diversity Ellesmere Island Lake A Phytoplankton Protists Taxonomy Water column ArcticNet Network of Centres of Excellence of Canada International Polar Year-Microbial Biodiversity of High Arctic Ecosystems |
spellingShingle |
Abundance Diversity Ellesmere Island Lake A Phytoplankton Protists Taxonomy Water column ArcticNet Network of Centres of Excellence of Canada International Polar Year-Microbial Biodiversity of High Arctic Ecosystems Vincent, Warwick Charvet, Sophie Veillette, Julie Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
topic_facet |
Abundance Diversity Ellesmere Island Lake A Phytoplankton Protists Taxonomy Water column ArcticNet Network of Centres of Excellence of Canada International Polar Year-Microbial Biodiversity of High Arctic Ecosystems |
description |
Protist samples were taken using a 6.2 L Kemmerer sampler, transferred to acid-washed, opaque 20 L plastic containers and stored in the dark at 4°C until processing. They were preserved with paraformaldehyde (0.1% final concentration) and glutaraldehyde (1% final concentration) in duplicate 50 mL polypropylene centrifuge tubes and stored at 4°C for up to 6 months in the dark until analysis; cellular features were well preserved and showed no evidence of degradation during storage. Protists were counted and identified using a combined system of fluorescence, Nomarski interference and Utermöhl sedimentation (FNU). 60 mL samples (16 mL for Lake A at 5 m on 24 August 2008, Lake B at 2 m on 24 August 2008 and Milne Fiord at 5 m on 13 July 2007) were concentrated in Utermöhl sedimentation chambers for 24 hours and stained with DAPI (0.1 mg/mL). Counts and identifications were made with a Zeiss Axiovert 100 inverted epifluorescence microscope under 400 and 1000X magnification (cells > 2 um in diameter). Cells were identified to genus wherever possible and were classified as heterotrophic when no chloroplast was observed. The microscopy detection limit was 2.67 x 10^3 cells/L. A minimum of 400 cells and 15 fields were counted wherever possible. : Purpose: This dataset present phytoplankton and other protist enumerations of Lake A, Lake B, Lake C1, Lake C2, Ward Hunt Lake and Milne Fiord at several depths in 2007 and 2008. The objective of this data was to evaluate the abundance and community composition of the phytoplankton by microscopy. This dataset is also useful to assess the implications for ecosystem structure and function of the increasingly observed loss of perennial lake ice. : Summary: Not Applicable |
format |
Dataset |
author |
Vincent, Warwick Charvet, Sophie Veillette, Julie |
author_facet |
Vincent, Warwick Charvet, Sophie Veillette, Julie |
author_sort |
Vincent, Warwick |
title |
Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
title_short |
Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
title_full |
Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
title_fullStr |
Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
title_full_unstemmed |
Protist enumerations from lakes and fiords along the northern coastline of Ellesmere Island (Ward Hunt Island observatory) |
title_sort |
protist enumerations from lakes and fiords along the northern coastline of ellesmere island (ward hunt island observatory) |
publisher |
Canadian Cryospheric Information Network |
publishDate |
2012 |
url |
https://dx.doi.org/10.5443/10927 https://www.polardata.ca/pdcsearch/?doi_id=10927 |
long_lat |
ENVELOPE(-74.161,-74.161,83.102,83.102) ENVELOPE(-81.447,-81.447,82.636,82.636) ENVELOPE(-125.856,-125.856,53.733,53.733) ENVELOPE(-100.601,-100.601,58.788,58.788) |
geographic |
Arctic Ellesmere Island Canada Ward Hunt Island Milne Fiord Island Lake Hunt Island |
geographic_facet |
Arctic Ellesmere Island Canada Ward Hunt Island Milne Fiord Island Lake Hunt Island |
genre |
Arctic ArcticNet Ellesmere Island International Polar Year Milne Fiord Phytoplankton Ward Hunt Island |
genre_facet |
Arctic ArcticNet Ellesmere Island International Polar Year Milne Fiord Phytoplankton Ward Hunt Island |
op_rights |
Public |
op_doi |
https://doi.org/10.5443/10927 |
_version_ |
1766345805007945728 |