Fatty acid composition of 32 cold-water algal strains cultured under different conditions to support the interpretation of in situ algal FA data from the MOSAiC expedition 2019/2020 ...

• Main Authors: Schmidt, Katrin, Graeve, Martin, Hoppe, Clara, Rokitta, Sebastian, Welteke, Nahid, Menendez, Cecilia Rad, Probert, Ian, Brenneis, Tina, Belt, Simon T., Atkinson, Angus
• Format: Dataset
• Language: English
• Published: NERC EDS UK Polar Data Centre 2023
• Subjects: "EARTH SCIENCE","BIOLOGICAL CLASSIFICATION","PLANTS","MICROALGAE","DIATOMS"; "EARTH SCIENCE","OCEANS","OCEAN CHEMISTRY","NUTRIENTS"; "EARTH SCIENCE","OCEANS","OCEAN TEMPERATURE","WATER TEMPERATURE"; "EARTH SCIENCE","OCEANS","SEA ICE"; Arctic; DHA; EPA; MOSAiC; Melosira arctica; cultured algae; fatty acids; light intensity; lipid biomarker; nutrients; sea ice; temperature; Arctic Ocean; Southern Ocean; Alfred Wegener Institute; North Atlantic; Sea ice
• Online Access: https://dx.doi.org/10.5285/bb4b1e74-d5af-4a73-9174-62a01807c641; https://data.bas.ac.uk/full-record.php?id=GB/NERC/BAS/PDC/01808

A seasonal cycle of the FA composition of particulate organic matter from surface waters, Chlorophyll-a maximum layer and bottom sea ice, sampled during the MOSAiC expedition in the Central Arctic Ocean (2019-2020), suggests the importance of phylogenetic and environmental drivers. To improve our understanding of these different drivers, we conducted culture experiments with 32 cold-water algal strains where temperature, light intensity, and nutrient supply were manipulated individually or in combination. The culture experiments were carried out at the Culture Collection of Algae and Protozoa (CCAP; Oban, Scotland), the Roscoff Culture Collection (RCC; Roscoff, France) and the Alfred-Wegener-Institute-Helmholtz-Centre for Polar and Marine Research (AWI; Bremerhaven, Germany). The strains were part of the culture collections, had been isolated in the Arctic (25 strains), Southern Ocean (2 strains) or North Atlantic (5 strains), and included diatoms, chlorophytes, haptophytes, cryptophytes, chrysophytes, ... : Microalgae cultures: In 2022, we conducted FA analysis on 32 cold-water strains that were isolated in the Arctic (25 strains), Southern Ocean (2 strains) or North Atlantic (5 strains), and included diatoms, chlorophytes, haptophytes, cryptophytes, chrysophytes, dinoflagellates and cyanobacteria. These strains were either obtained from commercial culture collections (CCAP or RCC) or provided by the AWI. The CCAP cultures were inoculated in 50 ml Erlenmeyer conical flasks with 25 ml of F/2 medium and added 60 micromol L-1 silicate for diatoms (Guillard and Ryther 1962). The algal strains were grown for four weeks at either low temperature - low light intensity (3-4 degrees Celsius, ~10 micromol m-2 s-1) or higher temperature - higher light intensity (8 degrees Celsius, 20 micromol m-2 s-1) and a light:dark cycle of 12:12 hrs. The culturing conditions at RCC resembled those at CCAP, but only for the low temperature - low light intensity (3-4d degrees Celsius, 10 micromol m-2 s-1). The algae growth rates were ...