Flow cytometry analysis of water samples for bacterial and Pico-plankton enumeration, samples collected in the Barents Sea during 2017-2018 ...

• Main Authors: Mitchell, Elaine, McNeil, Sharon, Whyte, Callum, Cottier, Finlo, Hopkins, Joanne, Davidson, Keith
• Format: Dataset
• Language: English
• Published: UK Polar Data Centre, Natural Environment Research Council, UK Research & Innovation 2021
• Subjects: "EARTH SCIENCE","BIOSPHERE","ECOLOGICAL DYNAMICS","ECOSYSTEM FUNCTIONS","BIOMASS DYNAMICS"; "EARTH SCIENCE","BIOSPHERE","ECOSYSTEMS","AQUATIC ECOSYSTEMS","PLANKTON"; "EARTH SCIENCE","OCEANS","MARINE ENVIRONMENT MONITORING"; Bacteria; Barents Sea; Flow cytometry; Pico-plankton; Arctic; Arctic Ocean
• Online Access: https://dx.doi.org/10.5285/2182fd8d-0a0d-4f56-9bf9-4dffaa67ff4b; https://data.bas.ac.uk/full-record.php?id=GB/NERC/BAS/PDC/01300

Collection and preservation of open ocean water samples from stations along a transect in the Barents Sea over the course of a year from July 2017 - July 2018. Four cruises in total to cover seasonal changes, two on board the James Clark Ross (RRS) and two aboard the Helmer Hansen (RV). A standard CTD cast was deployed to collect the samples, the depths were selected to support Primary Production experiments on board the ship, with deep samples representing 1% PAR. Research assistants from SAMS (Scottish Association for Marine Science) were responsible for the sample collection and Elaine Mitchell of SAMS was responsible for the sample analysis and data processing. This work was funded by Arctic PRIZE - NERC Thematic grant - Changing Arctic Ocean (CAO) programme - NE/P006302/1. ... : Sample collection: 4 cruises - JR16-006 / HH180101 / HH230418 / JR17-006 Seawater was collected from six depths from a standard environmental CTD cast as close to midday as possible. The CTD was positioned in full sunlight and not in the shadow of the ship. Sampling depths were selected based on the PAR irradiance readings from the CTD at the surface of the water (approx. 2m) after being initially stabilised at 10m and bought back to the surface.Set percentages of light 100%, 50%, 25%,15%,3% and 1% were calculated from the surface PAR and the depths chosen accordingly. For flow cytometry samples three depths were selected - Surface, Chlorophyll maximum and deep (exception was on HH180101 where only surface waters were taken due to it being wintertime). 10L acid washed carboys and acid washed tubing with 200 µm mesh to pre-screen the water were used to collect the water samples. The carboys were stored in black bags either in the cold room or on deck in a low light area. Location of the collected water for ...