Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide
Lipases from Candida antarctica and Mucor miehei were encapsulated in lecithin water-in-oil (w/o) microemulsion-based organogels (MBG). These gels were formulated with either hydroxypropylmethyl cellulose (HPMC) or gelatin. The esterification of lauric acid and 1-propanol catalyzed by these MBGs was...
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Universität Regensburg
2006
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ftdatacite:10.5283/epub.7 2023-05-15T13:39:22+02:00 Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide Blattner, Christian Zoumpanioti, Maria Kröner, Jürgen Schmeer, Georg Xenakis, Aristotelis Kunz, Werner 2006 https://dx.doi.org/10.5283/epub.7 https://epub.uni-regensburg.de/id/eprint/7 unknown Universität Regensburg http://dx.doi.org/10.1016/j.supflu.2005.06.007 http://dx.doi.org/10.1016/j.supflu.2005.06.007 https://dx.doi.org/10.1016/j.supflu.2005.06.007 Microemulsion; Organogels; Lipases; Enzymatic reaction; Supercritical CO2 530 Physik 540 Chemie Text Article article-journal ScholarlyArticle 2006 ftdatacite https://doi.org/10.5283/epub.7 https://doi.org/10.1016/j.supflu.2005.06.007 2021-11-05T12:55:41Z Lipases from Candida antarctica and Mucor miehei were encapsulated in lecithin water-in-oil (w/o) microemulsion-based organogels (MBG). These gels were formulated with either hydroxypropylmethyl cellulose (HPMC) or gelatin. The esterification of lauric acid and 1-propanol catalyzed by these MBGs was examined in supercritical carbon dioxide (scCO2; 35◦C, 110 bar) as solvent for the substrates. The results were compared to those obtained with the reference substrate solvent isooctane. It turned out that the initial rates of this model reaction in scCO2 were higher than those observed in the reference system. Various parameters affecting the biocatalysis such as pressure, alcohol and acid chain length, and gel composition were investigated. Kinetic studies showed that the ester synthesis catalyzed by the immobilized C. antarctica lipase occurs via a Ping Pong Bi Bi mechanism in which only inhibition by excess of alcohol was identified. Values of all kinetic parameters were determined. In addition, experiments on the reusability of these gels in scCO2 were carried out and the state of water within the organogel was examined with the help of differential scanning calorimetry. The present study shows that biocatalysis using MBGs in scCO2 is a promising alternative to other bioconversion processes. Text Antarc* Antarctica DataCite Metadata Store (German National Library of Science and Technology) |
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Microemulsion; Organogels; Lipases; Enzymatic reaction; Supercritical CO2 530 Physik 540 Chemie |
spellingShingle |
Microemulsion; Organogels; Lipases; Enzymatic reaction; Supercritical CO2 530 Physik 540 Chemie Blattner, Christian Zoumpanioti, Maria Kröner, Jürgen Schmeer, Georg Xenakis, Aristotelis Kunz, Werner Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
topic_facet |
Microemulsion; Organogels; Lipases; Enzymatic reaction; Supercritical CO2 530 Physik 540 Chemie |
description |
Lipases from Candida antarctica and Mucor miehei were encapsulated in lecithin water-in-oil (w/o) microemulsion-based organogels (MBG). These gels were formulated with either hydroxypropylmethyl cellulose (HPMC) or gelatin. The esterification of lauric acid and 1-propanol catalyzed by these MBGs was examined in supercritical carbon dioxide (scCO2; 35◦C, 110 bar) as solvent for the substrates. The results were compared to those obtained with the reference substrate solvent isooctane. It turned out that the initial rates of this model reaction in scCO2 were higher than those observed in the reference system. Various parameters affecting the biocatalysis such as pressure, alcohol and acid chain length, and gel composition were investigated. Kinetic studies showed that the ester synthesis catalyzed by the immobilized C. antarctica lipase occurs via a Ping Pong Bi Bi mechanism in which only inhibition by excess of alcohol was identified. Values of all kinetic parameters were determined. In addition, experiments on the reusability of these gels in scCO2 were carried out and the state of water within the organogel was examined with the help of differential scanning calorimetry. The present study shows that biocatalysis using MBGs in scCO2 is a promising alternative to other bioconversion processes. |
format |
Text |
author |
Blattner, Christian Zoumpanioti, Maria Kröner, Jürgen Schmeer, Georg Xenakis, Aristotelis Kunz, Werner |
author_facet |
Blattner, Christian Zoumpanioti, Maria Kröner, Jürgen Schmeer, Georg Xenakis, Aristotelis Kunz, Werner |
author_sort |
Blattner, Christian |
title |
Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
title_short |
Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
title_full |
Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
title_fullStr |
Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
title_full_unstemmed |
Biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
title_sort |
biocatalysis using lipase encapsulated in microemulsion-based organogels in supercritical carbon dioxide |
publisher |
Universität Regensburg |
publishDate |
2006 |
url |
https://dx.doi.org/10.5283/epub.7 https://epub.uni-regensburg.de/id/eprint/7 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://dx.doi.org/10.1016/j.supflu.2005.06.007 http://dx.doi.org/10.1016/j.supflu.2005.06.007 https://dx.doi.org/10.1016/j.supflu.2005.06.007 |
op_doi |
https://doi.org/10.5283/epub.7 https://doi.org/10.1016/j.supflu.2005.06.007 |
_version_ |
1766117813730148352 |