Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis
In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached living planctomycete. The development of an MLSA for Rhodop...
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ftdatacite:10.5283/epub.21388 2023-05-15T17:33:33+02:00 Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rossello-Mora, Ramon Harder, Jens 2010 https://dx.doi.org/10.5283/epub.21388 https://epub.uni-regensburg.de/id/eprint/21388 unknown Universität Regensburg https://dx.doi.org/10.1128/aem.01525-09 570 Biowissenschaften, Biologie Text Article article-journal ScholarlyArticle 2010 ftdatacite https://doi.org/10.5283/epub.21388 https://doi.org/10.1128/aem.01525-09 2021-11-05T12:55:41Z In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. Text North Atlantic DataCite Metadata Store (German National Library of Science and Technology) |
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570 Biowissenschaften, Biologie |
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570 Biowissenschaften, Biologie Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rossello-Mora, Ramon Harder, Jens Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
topic_facet |
570 Biowissenschaften, Biologie |
description |
In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species. |
format |
Text |
author |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rossello-Mora, Ramon Harder, Jens |
author_facet |
Winkelmann, Nadine Jaekel, Ulrike Meyer, Carolin Serrano, Wilbert Rachel, Reinhard Rossello-Mora, Ramon Harder, Jens |
author_sort |
Winkelmann, Nadine |
title |
Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
title_short |
Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
title_full |
Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
title_fullStr |
Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
title_full_unstemmed |
Determination of the Diversity of "Rhodopirellula" Isolates from European Seas by Multilocus Sequence Analysis |
title_sort |
determination of the diversity of "rhodopirellula" isolates from european seas by multilocus sequence analysis |
publisher |
Universität Regensburg |
publishDate |
2010 |
url |
https://dx.doi.org/10.5283/epub.21388 https://epub.uni-regensburg.de/id/eprint/21388 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://dx.doi.org/10.1128/aem.01525-09 |
op_doi |
https://doi.org/10.5283/epub.21388 https://doi.org/10.1128/aem.01525-09 |
_version_ |
1766132085764915200 |