Antibacterial potential of indigenous red mangrove (Rhizophora racemosa) fungal endophytes and bioactive compounds identification ...

• Main Authors: C. N. Ariole, A. E. Akinduyite
• Format: Article in Journal/Newspaper
• Language: English
• Published: Zenodo 2016
• Subjects: Rhizophora racemosa; Marine bacterial pathogens; Antibacterial activity; Fungal endophytes; Eagle Island
• Online Access: https://dx.doi.org/10.5281/zenodo.8371596; https://zenodo.org/record/8371596

The antibacterial activity of indigenous red mangrove leaves- endophytic fungi against marine pathogenic bacteria and bioactive compounds identification and quantification were carried out. Healthy leaves of red mangrove ( Rhizophora racemosa) were collected from Eagle Island mangrove forest in Port Harcourt, Nigeria. The endophytic fungi were isolated with acidified Potato dextrose agar plates after incubation at 28°C for 5 days. Isolated colonies were identified based on their colonial and microscopic morphology. The ethyl acetate and methanolic extracts of the mangrove leaves-endophytic fungal isolates were screened against marine pathogenic bacteria ( Staphylococcus aureus , Salmonella sp., Shigella sp., Vibrio cholerae and Vibrio parahaemolyticus ) using agar well diffusion assay at different concentrations. An isolate which was active against all the pathogens was further identified molecularly and the bioactive compounds in its methanolic extract identified and quantified using Gas chromatography – ... : {"references": ["Abhiroop C, Pranabes S, Subodh KM. 2014. Ethnobotanical understanding of Mangrove. Interna-tional Journal of Botany and Research 4, 29-34.", "Alias SA, Zainuddin N, Lee CW, Ebel R, Othman NA, Mukhtar MR, Awang K. 2010. Antimicrobial activities of marine fungi from Malaysia. Botanica Marina 53, 507-513.", "Alvin A, Miller KI, Neilan BA. 2014. Exploring the potential of Endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiology Research 169, 483-495.", "Ariole CN, Ihunwo HO, Ozuru CN. 2014. Isolation of Marine Green Alga \u2013 Associated Microorganisms and their Antimicrobial activity against Fish Pathogenic Aeromonas Strain. Journal of Chemical,Biological and Physical Sciences 4(4), 3279-3286.", "Bhimba BV, Agnel DA, Mathew JM, Jose GM, Joel EL, Thangaraj M. 2012. Anticancer and antimicrobial activity of Mangrove derived fungi Hypocrea lixii. Chinese Journal of Natural Medicines 10, 77-80.", "Chaeprasert S, Piapukiew AJ, Sihanonth P. 2010. Endophytic fungi from ...