ERK activity in migrating MDA-MB-231 cells (clover-ERK-KTR + sir-DNA) ...

• Main Authors: Pylvänäinen, Joanna W, Conway, James RW, Jacquemet, Guillaume
• Format: Dataset
• Language: unknown
• Published: Zenodo 2023
• Subjects: ERK; cell migration; MDA-MB-231; live cell imaging; Orca
• Online Access: https://dx.doi.org/10.5281/zenodo.8336015; https://zenodo.org/record/8336015

MDA-MB-231 or U2OS cells stably expressing clover-ERK-KTR were seeded on fibronectin-coated (1 µg /ml) Ibidi 8-well slides (Ibidi) 1 day before imaging. Four hours before imaging, the medium was supplemented with 250 nM sir-DNA (Cytoskeleton) and 25 mM HEPES (Sigma). Cells were then imaged live (37 °C, 5% CO 2 ) using a Nikon Eclipse Ti2-E microscope (Nikon) equipped with an sCMOS Orca Flash4.0 camera (Hamamatsu) and controlled by the NIS-Elements software (Nikon, v 5.11.01). MDA-MB-231 cells were imaged using a 20× Nikon CFI Plan Apo Lambda objective (NA 0.75), either 1 frame per minute for 2 hours or 1 frame every 5 minutes for 17 hours. In these experiments, a camera binning of 2 × 2 was used. This dataset consists of 20 videos. ...