Imagesurf Moab2 Image Example

• Main Authors: O'Mara, Aidan R, Collins, Jessica M, King, Anna E, Vickers, James C, Kirkcaldie, Matthew T K
• Format: Dataset
• Language: unknown
• Published: Zenodo 2017
• Subjects: Segmentation; MOAB2; Confocal imaging; Orca
• Online Access: https://dx.doi.org/10.5281/zenodo.819511; https://zenodo.org/record/819511

A set of 2000x2000 raw confocal fluorescence images of MOAB2-labelled cortex from APPswe/PS1dE9 mouse models of Alzheimer's disease, un-annotated intensity scaled 8-bit images, sparsely annotated pixel labels and reference segmentation examples. Pixels are annotated as signal (red 0xFFFF0000) and background (blue 0xFF0000FF). Images were captured as stitched 12-bit greyscale single-plane images and cropped to size. Image acquisition was performed at 561nm excitation and 615nm emission wavelengths using a Perkin Elmer Ultraview VOX imaging system with Nikon Eclipse Ti inverted microscope, 20x objective lens, x-y motorised stage, Hamamatsu ORCA-R2 C10600-10B CCD camera and Volocity 6.3 software package. These images are shared as examples for the ImageSURF segmentation plugin for ImageJ2/FIJI (https://doi.org/10.5281/zenodo.818109).