Imagesurf Moab2 Image Example

• Main Authors: O'Mara, Aidan R, Collins, Jessica M, King, Anna E, Vickers, James C, Kirkcaldie, Matthew T K
• Format: Text
• Language: unknown
• Published: Zenodo 2017
• Subjects: Segmentation; MOAB2; Confocal imaging; Orca
• Online Access: https://dx.doi.org/10.5281/zenodo.787849; https://zenodo.org/record/787849

A set of 2000x2000 confocal fluorescence images of MOAB2-labelled cortex from APPswe/PS1dE9 mouse models of Alzheimer's disease, sparsely annotated pixel labels and reference segmentation examples. Pixels are annotated as signal (red 0xFFFF0000) and background (blue 0xFF0000FF). Images were captured as stitched 12-bit greyscale single-plane images and cropped to size. Image acquisition was performed at 561nm excitation and 615nm emission wavelengths using a Perkin Elmer Ultraview VOX imaging system with Nikon Eclipse Ti inverted microscope, 20x objective lens, x-y motorised stage, Hamamatsu ORCA-R2 C10600-10B CCD camera and Volocity 6.3 software package. These images are shared as examples for the ImageSURF segmentation plugin for ImageJ2/FIJI (https://doi.org/10.5281/zenodo.375947).